PhyNexus's PhyTip Columns
PhyNexus's PhyTip Columns

ProPlus / ProPlus LX PhyTip Columns (MabSelect SuRe™)

2020-12-02
조회수 174

The ProPlus (MabSelect SuRe™) and ProPlus LX (MabSelect SuRe™ LX) resin ligand is a recombinant Protein A derived ligand that demonstrates attributes of both Protein A and Protein G. ProPlus and ProPlus LX share a number of features including a modified Protein A ligand designed for alkali stability. Also, the modified Protein A is coupled to the resin matrix in an orientation that maximizes capacity. Choose ProPlus LX for high titer cultures to take advantage of high dynamic binding capacity. In certain cases, Protein G affinity resin will still demonstrate  superior selectivity over Protein A such as for certain isotypes and species of IgGs, including some mouse and rat IgGs.

ProPlus and ProPlus LX

  • Modified Protein A increases selectivity for Mouse IgGs
  • Optimal capacity due to ligand orientation
  • Capture, purify and enrich in as little as 15 minutes to obtain high concentrations of fully functional protein
  • Process small sample volumes in a reproducible, high throughput, automated format
  • Elution volumes as low as 10 μL, producing enrichment factors as high as 50 fold,  with concentrations of purified protein of up to 10 mg/mL
  • At least 10% higher capacity than Protein A resins
  • Broad range of selectivity, recognizing various isotypes and species, similar to Protein G
  • 3-5 fold greater recovery than Protein G

ProPlus LX

  • High dynamic binding capacity for high titer cultures


ProPlus / ProPlus LX columns with our proprietary Dual Flow Chromatography provide complete antibody binding and high final concentrations. PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent Technologies, Beckman Coulter, Dynamic Devices, HamiltonOpentrons, Perkin Elmer, and Tecan.

MabSelect SuRe and MabSelect SuRe LX are trademarks of GE Healthcare.