The ProPlus (MabSelect SuRe™) and ProPlus LX (MabSelect SuRe™ LX) resin ligand is a recombinant Protein A derived ligand that demonstrates attributes of both Protein A and Protein G. ProPlus and ProPlus LX share a number of features including a modified Protein A ligand designed for alkali stability. Also, the modified Protein A is coupled to the resin matrix in an orientation that maximizes capacity. Choose ProPlus LX for high titer cultures to take advantage of high dynamic binding capacity. In certain cases, Protein G affinity resin will still demonstrate superior selectivity over Protein A such as for certain isotypes and species of IgGs, including some mouse and rat IgGs.
ProPlus and ProPlus LX
- Modified Protein A increases selectivity for Mouse IgGs
- Optimal capacity due to ligand orientation
- Capture, purify and enrich in as little as 15 minutes to obtain high concentrations of fully functional protein
- Process small sample volumes in a reproducible, high throughput, automated format
- Elution volumes as low as 10 μL, producing enrichment factors as high as 50 fold, with concentrations of purified protein of up to 10 mg/mL
- At least 10% higher capacity than Protein A resins
- Broad range of selectivity, recognizing various isotypes and species, similar to Protein G
- 3-5 fold greater recovery than Protein G
ProPlus LX
- High dynamic binding capacity for high titer cultures
ProPlus / ProPlus LX columns with our proprietary Dual Flow Chromatography provide complete antibody binding and high final concentrations. PhyTip columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent Technologies, Beckman Coulter, Dynamic Devices, Hamilton, Opentrons, Perkin Elmer, and Tecan.
MabSelect™ SuRe and MabSelect™ SuRe LX are trademarks of GE Healthcare.
