FAQs

2025-08-07
조회수 227


  • What are DTG syringe columns used for?
    DTG syringe columns are used for purifying proteins and antibodies—specifically His-tagged proteins (IMAC) and IgG antibodies (ProA)—without the need for centrifuges, pumps, or magnetic setups


  • Do I need any special equipment to use them?
    No special equipment is required. Just connect the syringe to your sample and buffers—everything is controlled manually using a standard 5 mL syringe.


  • How much protein or antibody can I purify?

    • IMAC columns can purify up to 7 mg of His-GFP.

    • ProA columns recover 74–76% IgG from serum or culture supernatants.


  • Can the columns be reused?
    Yes! Both IMAC and ProA syringe columns can be reused up to 10 times with consistent performance when properly handled and stored.


  • What makes syringe columns better than spin columns or magnetic beads?
    DTG syringe columns combine high yield, manual control, and reusability, while eliminating the need for expensive equipment or single-use formats—making them faster and more cost-effective.


  • What volume do the columns handle?
    Each column contains 100 µL of resin, ideal for processing up to 1 mL of sample.


  • How do I store the columns after use?
    Just cap the syringe and store it at 4 °C in buffer. The resin remains hydrated—no drying or repacking required.


  • Can I try them before buying?
    Yes! We offer free demo packs that include one IMAC and one ProA syringe column. You just cover shipping.


  • How does the syringe work?