A rapid and extraction-free diagnostic platform that detects ASFV within 20 min.

A rapid and extraction-free diagnostic platform that detects ASFV within 20 min.

sCRAM integrates a rapid nucleic acid release step (40◦C, 5 min) with a one-tube reaction combining Multienzyme Isothermal Rapid Amplification (MIRA) and a suboptimal PAM-mediated CRISPR/Cas12a system (37◦C, 15 min), enabling instrument-free visual readout via UV light or lateral flow strips (LFS).

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