Super-Fast Detection Of Bacillus Cereus By Combining Cellulose Filter Paper-Based DNA Extraction,MIRA-LFD
Jan 20, 2026 View: 106 Leave a message
Recently, a well-known infant formula company proactively initiated a preventive recall of its infant formula worldwide after Bacillus cereus was detected in its raw materials. How to detect potential hazards more quickly, timely, and accurately has become a common concern for both upstream and downstream sectors of the industry chain.
MIRA-LFD provides a technical solution for on-site rapid detection of Bacillus cereus, completing a full testing process in 25 minutes, efficiently and accurately! It can be flexibly applied in various scenarios, including production sites, supply chain traceability, and customs inspection.
The journal Foods published an article titled 'Super-Fast Detection of Bacillus cereus by Combining Cellulose Filter Paper-Based DNA Extraction, Multienzyme Isothermal Rapid Amplification, and Lateral Flow Dipstick (MIRA-LFD)'.
Inthis study, a simple and super-fast method for detecting B. cereus was developed, which combines cellulose filter paper-based DNA extraction, multienzyme Isothermal Rapid Amplification (MIRA), and lateral flow dipstick (LFD) technology. Initially, PCR was adopted to evaluate the DNA extraction efficiency of the filter paper, followed by the optimization of the lysis formula and extraction conditions. With the above optimization,
DNA that can be used for subsequent nucleic acid amplification can be obtained within 3 min. Then, the isothermal amplification of MIRA–LFD was established and optimized to evaluate the detection specificity and sensitivity. Finally, the developed method was applied to detect B. cereus in cooked rice samples. The results indicated that the entire amplification procedure of MIRA-LFD only takes 15 min at 39 ◦C. The whole super-fast detection system could be completed in less than 20 min, from DNA extraction to result interpretation,which achieved a detection limit of 12 fg/µL of DNA concentration, corresponding to approximately 115 CFU/mL in actual samples.
In this study, cellulose filter paper-based DNA extraction in combination with MIRA–LFD was used for visual detection of B. cereus. The detection can be completed in less than 20 min from DNA extraction to result determination. This study combined DNA extraction and rapid amplification, and the results could be observed by the naked eye without using any large-scale instrument, which is easy to operate, rapid, and consistent with the field testing outside of the laboratory.
In this study, MIRA DNA Isothermal Rapid Amplification Kit(Nucleic Acid Test Strip ) and Nucleic Acid Test Strip was used.
MIRA DNA Isothermal Rapid Amplification Kit(Nucleic Acid Test Strip ):
- Constant temperature amplification for 20 minutes, fast and efficient, minimal equipment dependence
- Detection limit of 1-5 copies/μL, high specificity
- Lyophilized reagents, easy to use, can be stored and transported at room temperature
- Various reagent forms to meet different operational needs
Nucleic Acid Test Strip:
- Single/Dual /Triple Detection
- Optimized based on MIRA methodology, more adaptable
- Combining MIRA technology, highlighting convenience advantage
MIRA (Multienzyme Isothermal Rapid Nucleic Acid Amplification) technology is ideally suited for on-site rapid nucleic acid detection.
MIRA can achieve rapid nucleic acid detection in 5–20 minutes. It is easy to operate and does not require complex instruments, providing a more flexible and accurate technical method and overall solution for on-site rapid testing.
Super-Fast Detection Of Bacillus Cereus By Combining Cellulose Filter Paper-Based DNA Extraction,MIRA-LFD
Jan 20, 2026 View: 106 Leave a message
Recently, a well-known infant formula company proactively initiated a preventive recall of its infant formula worldwide after Bacillus cereus was detected in its raw materials. How to detect potential hazards more quickly, timely, and accurately has become a common concern for both upstream and downstream sectors of the industry chain.
MIRA-LFD provides a technical solution for on-site rapid detection of Bacillus cereus, completing a full testing process in 25 minutes, efficiently and accurately! It can be flexibly applied in various scenarios, including production sites, supply chain traceability, and customs inspection.
The journal Foods published an article titled 'Super-Fast Detection of Bacillus cereus by Combining Cellulose Filter Paper-Based DNA Extraction, Multienzyme Isothermal Rapid Amplification, and Lateral Flow Dipstick (MIRA-LFD)'.
Inthis study, a simple and super-fast method for detecting B. cereus was developed, which combines cellulose filter paper-based DNA extraction, multienzyme Isothermal Rapid Amplification (MIRA), and lateral flow dipstick (LFD) technology. Initially, PCR was adopted to evaluate the DNA extraction efficiency of the filter paper, followed by the optimization of the lysis formula and extraction conditions. With the above optimization,
DNA that can be used for subsequent nucleic acid amplification can be obtained within 3 min. Then, the isothermal amplification of MIRA–LFD was established and optimized to evaluate the detection specificity and sensitivity. Finally, the developed method was applied to detect B. cereus in cooked rice samples. The results indicated that the entire amplification procedure of MIRA-LFD only takes 15 min at 39 ◦C. The whole super-fast detection system could be completed in less than 20 min, from DNA extraction to result interpretation,which achieved a detection limit of 12 fg/µL of DNA concentration, corresponding to approximately 115 CFU/mL in actual samples.
In this study, cellulose filter paper-based DNA extraction in combination with MIRA–LFD was used for visual detection of B. cereus. The detection can be completed in less than 20 min from DNA extraction to result determination. This study combined DNA extraction and rapid amplification, and the results could be observed by the naked eye without using any large-scale instrument, which is easy to operate, rapid, and consistent with the field testing outside of the laboratory.
In this study, MIRA DNA Isothermal Rapid Amplification Kit(Nucleic Acid Test Strip ) and Nucleic Acid Test Strip was used.
MIRA DNA Isothermal Rapid Amplification Kit(Nucleic Acid Test Strip ):
Nucleic Acid Test Strip:
MIRA (Multienzyme Isothermal Rapid Nucleic Acid Amplification) technology is ideally suited for on-site rapid nucleic acid detection.
MIRA can achieve rapid nucleic acid detection in 5–20 minutes. It is easy to operate and does not require complex instruments, providing a more flexible and accurate technical method and overall solution for on-site rapid testing.