Multienzyme Isothermal Rapid Amplification(MIRAmp)(RPA) Kits & MIRA Technology
Multienzyme Isothermal Rapid Amplification(MIRAmp)(RPA) Kits & MIRA Technology
Development and Evaluation of Duplex MIRA-qPCR Assay for Simultaneous Detection of Staphylococcus aureus and non-aureus Staphylococci
The primers and probes were synthesized and purified by Sangon Bioengineering (Shanghai, China) Co., Ltd.
Wizard Genomic DNA Extraction Kit (Promega, Madison, WI, USA) was used to extract DNA according to the manufacturer’s protocol.
MIRA-qPCR amplification was performed by Applied Biosystems QuantStudio 6 Flex Real-Time PCR (Singapore Life Technologies Holdings Pte Ltd., Singapore). MIRA basic kit (Amp-future, Weifang, China) was used for nucleic acid amplification according to the manufacturer’s instructions.