BioToolomics' Process Chromatography Media
BioToolomics' Process Chromatography Media

Hydrophobic Interaction Chromatography (HIC) Media

Hydrophobic Interaction Chromatography (HIC) Media 제품 문의 

HIC is a versatile technique and can show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications.

Butyl SepFast™, Phenyl SepFast™, and Octyl SepFast™ are hydrophobic interaction chromatography (HIC) adsorbents with carbon chains of C4, C6 (benzene ring), and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.

HIC media requires much milder purification conditions than reversed phase chromatography (RPC) media. Hence biological activity can be maintained in HIC separations unlike RPC operations.

HIC media normally binds at moderate to high salt concentrations. It is therefore logical to place a HIC step after an IEX step where molecules are usually eluted at high salt conditions.

SepFast™ HIC media offers a broad range of choices from C4 to C8. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity. 

Biotoolomics HIC media gives you:

  • High sample loading capacity
  • High separation power
  • Available at all pack sizes from analytical up to full manufacturing scale.
  • Chemically stable Agarose base matrix.
  • Can be produced in high and low ligand densities, alternate crosslinking levels and different bead sizes to optimise the media to the separation.
  • All versions can be scaled up and produced for GMP use
  • More choices than other suppliers.

The feature and selection guide is listed as follows:

4HF serial
(50µm - 150µm)
6HF serial
(50µm - 150µm)
Large Beads
(150µm - 350µm)
(25µm - 50µm)
The above HIC media is designed to purify most medium to large proteinsThe above HIC media is designed to purify peptides or smaller proteinsThe above HIC media is designed to purify proteins from crude or viscous samplesThe above HIC media is designed to purify proteins
that require high resolution

Characteristics of SepFast HIC Media

MatrixHighly cross-linked agarose; 4% agarose for SepFast 4HF, 6% agarose for SepFast 6HF
Around 40 µmol/mlAround 25 µmol/ml;
Around 40 µmol/ml (high sub)
Around 4 µmol/ml
>20 mg lyzosyme / ml>20 mg BSA / ml>20 mg BSA / ml
25 - 50 µm (HighRes), 50 - 150 µm (4HF and 6HF), 150 - 350 µm (Large Beads)
>100 cm/h for HighRes; >300 cm/h for 4HF serials; >500 cm/h for 6HF serials; >1500 cm/h for Large Beads
Up to 3 bar
2-14 (short term) and 3-12 (long term)
4oC – 30oC
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 2 M (NH4)2SO4
Storage20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

DescriptionQuantityCode no.Price GBP
HIC SepFast Selection Kit7 x 1 ml column packed with Butyl 4HF, Butyl 6HF, Phenyl 4HF, Phenyl 6HF, Phenyl 6HF high sub, Octyl 4HF and Octyl 6HF, respectively480000-7x1ML£183.75

SepFast Butyl Products

SepFast Phenyl ProductsSepFast Octyl Products
DescriptionQuantityCode no.Price GBP
Butyl SepFast 4HF25 ml480101-25ML£99.67
 100 ml480101-100ML£262.51
 1 Litre480101-1LPOR
Butyl SepFast 4HF prepacked column5 x 1 ml480101-5x1ML£135.45
 5 x 5 ml480101-5x5ML£281.40
Butyl SepFast 6HF25 ml480301-25ML£99.67
 100 ml480301-100ML£262.51
 1 Litre480301-1LPOR
Butyl SepFast 6HF prepacked column5 x 1 ml480301-5x1ML£135.45
 5 x 5 ml480301-5x5ML£281.40
Butyl SepFast Large Beads25 ml480201-25ML£99.67
 100 ml480201-100ML£262.51
 1 Litre480201-1LPOR
SepFast Butyl Products

SepFast Phenyl Products

SepFast Octyl Products
DescriptionQuantityCode no.Price GBP
Phenyl SepFast 4HF25 ml481001-25ML£99.67
 100 ml481001-100ML£262.51
 1 Litre481001-1LPOR
Phenyl SepFast 4HF prepacked column5 x 1 ml481001-5x1ML£135.45
 5 x 5 ml481001-5x5ML£281.40
Phenyl SepFast 6HF25 ml481201-25ML£99.67
 100 ml481201-100ML£262.51
 1 Litre481201-1LPOR
Phenyl SepFast 6HF prepacked column5 x 1 ml481201-5x1ML£135.45
 5 x 5 ml481201-5x5ML£281.40
Phenyl SepFast 6HF High sub25 ml481202-25ML£99.67
 100 ml481202-100ML£262.51
 1 Litre481202-1LPOR
Phenyl SepFast 6HF High sub prepacked column5 x 1 ml481202-5x1ML£135.45
 5 x 5 ml481202-5x5ML£281.40
Phenyl SepFast HighRes25 ml481101-25ML£120.75
 100 ml481101-100ML£315.00
 1 Litre481101-1LPOR
Phenyl SepFast HighRes prepacked column5 x 1 ml481101-5x1ML£161.70
 5 x 5 ml481101-5x5ML£308.70
Phenyl SepFast Large Beads25 ml481301-25ML£99.67
 100 ml481301-100ML£262.51
 1 Litre481301-1LPOR
SepFast Butyl ProductsSepFast Phenyl Products

SepFast OctylProducts

DescriptionQuantityCode no.Price GBP
Octyl SepFast 4HF25 ml481601-25ML£99.67
 100 ml481601-100ML£262.51
 1 Litre481601-1LPOR
Octyl SepFast 4HF prepacked column5 x 1 ml481601-5x1ML£135.45
 5 x 5 ml481601-5x5ML£281.40
Octyl SepFast 6HF25 ml481801-25ML£99.67
 100 ml481801-100ML£262.51
 1 Litre481801-1LPOR
Octyl SepFast 6HF prepacked column5 x 1 ml481801-5x1ML£135.45
 5 x 5 ml481801-5x5ML£281.40
Octyl SepFast Large Beads25 ml481701-25ML£99.67
 100 ml481701-100ML£262.51
 1 Litre481701-1LPOR

Note: HIC media with lower or higher ligand density can be custom-made to increase recovery or binding capacity. Please contact us for more information.