Mixed or multi-modal ligands constitute a new group of ligands that share the ability to interact with target molecules through multiple types of interactions. In addition to ionic interactions, several other types of interactions are involved such as hydrogen bonding, hydrophobic interaction and p-p interaction. The strength of the individual interactions depends on the overall binding conditions.
Media containing mixed mode ligands possesses selectivities that are different from those of the “traditional” ligands. This type of media is generally more salt-tolerant and could be more selective than the ion exchange or hydrophobic media alone.
The first mixed mode product we offer is SepFast MM AH-1. It contains a combination of anionic and hydrophobic groups. In connection with the NCAP technology, an array of other mixed mode ligand products with complimentary functionalities will be introduced.
SepFast MM AH-1 is a mixed mode chromatography medium that is supplied in a ready-to-use disposable format. The ligand contains a combination of anionic and hydrophobic groups. The product shows good binding capacity to molecules rich in hydrophobic zones.
The base matrix is made of cross-linked porous polysaccharide. The particle has open pore structure with good mass transfer property. The particle size is 50 - 150µm. The medium is compatible with most of the chemical reagents commonly used in biological systems.
The medium is stored in 20% industrial ethanol at 4-30oC. Before a chromatography run, equilibrate the column with working buffer until the effluent shows stable conductivity and pH value.
The operating pressure is 4 bar or less.
SepFast MM AH-1 can be used for the capturing of proteins in a binding-elution mode. It can also be used in a flow-through mode to remove the impurities. We recommend scouting for the optimal binding, washing and elution conditions such as pH and conductivity.
|Product||Quantity*||Code no.||Price GBP|
|SepFast MM AH-1, 0.01 ml||50 SuperSpin Micro columns||270101||POR|
|SepFast MM AH-1, 1 ml||5 columns||270102||£108.05|
|SepFast MM AH-1, 5 ml||5 columns||270103||£439.90|
*Other volume sizes are available on request.