BioToolomics' Process Chromatography Media
BioToolomics' Process Chromatography Media

SepFast Gel Filtration Media

SepFastTM Gel Filtration Media 제품 문의


SepFast gel filtration media is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Gel filtration (or called size exclusion chromatography) is a proven technique, which is widely used for size-based molecular separations.

SepFast gel filtration media has a balanced design to offer high recovery and high selectivity according to individual applications. The core advantages are:

  • High resolution
  • High selectivity
  • High recovery
  • Highly scalable
  • Excellent physical and mechanical stability
  • Cost effective

The base matrix is made of agarose or a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

The selection guide is listed as follows:

                              SepFast GF-HS
 SepFast GF-HS-MSepFast GF-HS-L
Separation range3x103 - 7x1046x103 - 6x105
ApplicationPreparative grade separation for research, process development and large-scale productions
Particle Size(µm)      20-50     20-50
Format supplied      Loose resin and pre-packed columns

References Featuring Biotoolomics SepFast GF-HS-L Products:

de Cassan, S.C., Shakri, A.R., Llewellyn, D., Elias, S.C., Cho, J.S., Goodman, A.L., Jin, J., Douglas, A.D., Suwanarusk, R., Nosten, F.H. and Rénia, L., 2015. Preclinical assessment of viral vectored and protein vaccines targeting the Duffy-binding protein region II of Plasmodium vivax. Frontiers in immunology, 6, p.348

Jin, J., Tarrant, R.D., Bolam, E.J., Angell-Manning, P., Soegaard, M., Pattinson, D.J., Dulal, P., Silk, S.E., Marshall, J.M., Dabbs, R.A. and Nugent, F.L., 2018. Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5. 1 protein vaccine expressed in Drosophila S2 cells. NPJ vaccines, 3(1), p.32

Characteristics of  Gel Filtration Media

MatrixHighly cross-linked agarose and dextran composites
Particle sizeAround 35 µm  (in the range of 20 – 50 µm)
Operational pressure3 bar (0.3 MPa, 42 psi) for SepFast GF-HS-L
4 bar (0.4 Mpa, 56 psi) for SepFast GF-HS-M
Operating flow velocityNormally 10-50 cm/hour
pH stability2-14 (short term) and 3-12 (long term)
4oC – 30oC
Chemical stabilityAll commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
AvoidOxidizing agents
StorageThe media should be stored in 20% ethanol to prevent microbial growth. Store the media at a temperature of +2oC to +8oC.

Ordering information

DescriptionQuantityCode no.Price GBP
SepFast GF-HS-M
(SepFast SEC 3-70KDa prep grade)
25 ml540201-25ML£120.39
 150 ml540201-150ML£436.49
 1 Litre540201-1LPOR
 We also provide other bulk volumes. Please contact us for further information.
SepFast GF-HS-M column16 x 600 mm3-70-16x600£1383.42
 26 x 600 mm3-70-26x600POR
 We also provide pre-packed columns with other diameters and heights. Please contact us for further information
SepFast GF-HS-L
(SepFast SEC 6-600KDa prep grade)
25 ml540101-25ML£120.39
 150 ml540101-150ML£436.49
 1 Litre540101-1LPOR
 We also provide other bulk volumes. Please contact us for further information.
SepFast GF-HS-L column16 x 600 mm6-600-16x600£1383.42
 26 x 600 mm6-600-26x600POR
 We also provide pre-packed columns with other diameters and heights. Please contact us for further information