e-BLOT Western Blot Imaging Systems

2021-11-10
조회수 281

기기데모가능(요청/문의) : biotools@icloud.com

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 2. 샘플을 별도 준비하면서 테스트 경우 : 월요일 설치~ 수요일 철수 / 수요일 설치 ~ 금요일 철수



Struggling to Get Accurate, Reliable and Replicable Western Blot Results?

Integral to the study of cellular proteins is the effective discernment and qualification of many proteins amongst multiple cell lines. While traditional western blots provide this information, they require specific calibrations to reveal dark and faint bands and would take multiple tries to get the proper data needed, consuming unnecessary time and effort. Additionally, traditional western blot imaging systems capture strong signals from nearby bands along with the target protein, turning the task of capturing its data a bigger and time-consuming challenge.



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X-ray Film Western Blot

Functioning similarly to an old-fashioned darkroom photograph, the image of the protein sample is captured using a high-powered x-ray machine. The procedure has a high sensitivity rate and the finished image has no optical signal loss.While the method is still preferred in countries like the US, the use of x-ray film in western blotting has its share of downsides:

  • High maintenance and overhead costs, as the procedure requires a dark room, reagents, and other chemicals.
  • The chemicals required for processing the x-ray film are harmful to both the human body and the environment.
  • Preparing and processing the x-ray film consumes a significant amount of time and manpower.
  • Its weak signal acquisition, narrow dynamic range, and images that are prone to saturation results into low-accuracy images that could require multiple takes.

Cooled CCD Western Blot

Utilizing industrial-grade SLR cameras to capture the required images, the cooled CCD western blot method is making itself known in the field as an alternative to x-ray film. Compared to the traditional x-ray film western blot method, the cooled CCD process boasts accurate quantitative imaging, a wider range of images, and digital documentation, among others.

Despite its set of advantages, the cooled CCD western blot imaging system has its own set of setbacks, which include:

  • Low sensitivity and a high optical signal loss of more than 99%
  • A narrow quantitative range that could limit the desired results of the western blot imaging process.
  • Images are prone to saturation, resulting in poor and unusable images, therefore requiring more time to produce the right images.



A Better Approach To Western Blot Imaging





Touch Imaging, The Complete Revolution Against Traditional Imaging Technology

Contact chemiluminescence imaging is an imaging process in which the lens is removed, allowing the sample to direct contact with the photographic chip so the imaging process can be done in just 1 second. Moreover, the sensor chip area is 130 times larger than the conventional cold CCD imaging, and the individual pixel size area is also increased by over 400 times.

 

 

This innovative design eliminates many bottlenecks of traditional cold CCD imaging:

 

  • The western film is directly attached to the photographic chip for imaging, all signals are captured without any light loss, which greatly improves the sensitivity and speed of imaging;

 

  • The super large size of the photographic chip with ultra high signal acquisition capacity improves the sensitivity and speed of imaging;

 

  • The huge pixels can increase the quantification range by two orders of magnitude, enabling precise quantification of samples;

 

  • The new imaging technology removes any lens for imaging, thus eliminates image distortion caused by the lens and provides more accurate results.




Ultra High Sensitivity

The direct touch imaging system with no lens captures all signals without losing even the weakest bands. The super large size of the photographic chip also improves sensitivity that enables more accurate results.


Broader Dynamic Range

The new technology allows the samples for direct contacting the chip, which significantly increased the sensitivity by 2-3 logs for even the weakest signals, thus greatly reducing chances of signal loss or imaging failure.


Imaging in Just 1 Second

The whole imaging process is designed to be as simplified as possible to save your time in the lab. Just loading the sample onto the chip and press the imaging button, your imaging result is available right away.


Save Up to 90% Space

Using the latest technology available, our Touch Imager features a compact design that is sure to fit laboratories big or small. Its all-in-one solution additionally eliminates the need for other equipment or dedicated rooms, further saving space on the location.



The Imaging Technology That Performs Better In All Scenarios



TOUCH IMAGER VS. X-ray film





TOUCH IMAGER VS. cooled CCD





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Touch Imager

Get accurate images every time with our Touch Imager that takes advantage of large-scale electronic light-sensitive elements to produce clear and desirable protein images.

Highlights
  • Real dynamic collection range and large full-well electron capacity provides high overexposure resistance
  • Modular software adopts to the latest application, requiring less equipment
  • Easy analysis of exposure quantifies the exact signal needed on the bends
  • NASA-class sensors guarantee clear and crisp images every time
  • Stable and smart construction allows the Touch Imager to function without the need for cooling systems.