Miami Orange is a DNA-binding fluorescent probe that allows for two-channel imaging of nuclear and cytosolic compartments.

Highlights:

• Low cytotoxicity, membrane permeable, and live-cell compatible fluorescent probe
• Brightly fluorescent 'turn-on' emission when bound to DNA
• Cell permeable - rapidly accumulats intracellularly into cell nuclei and cytosol
• Differentiates between DNA and other cellular binding sites through shifts in excitation and/or emission spectra
• Two-color resolution - allows for seperation of cytosolic and nuclear populations into two imaging channels
• Minimal spectral overlap with common tags such as blue, cyan or greenfluorescent proteins
• Compatible with standard laser lines (e.g., 458, 488, 514, 561 nm) or filter sets (e.g., GFP, FITC, YPF)

The Miami Orange and Miami Red dyes are DNA-targeting probes that exhibit binding-induced ‘turn on’ fluorescence. While a ‘turn-on’ response can result through interaction with RNA or any number of protein binding folds present in the cytosolic milieu, these probes bound to DNA exhibit redder excitation and emission spectra, enabling clear resolution of the nucleus. These dyes effectively combine the function of two separate dyes (i.e., a nuclear stain and a cytosolic stain) in a single probe.

From the laboratory of James N. Wilson, PhD, University of Miami.