QuickPick™ IMAC
62301 • metal affinity kit for proteins, 8 preps 62311 • metal affinity kit for proteins, 48 preps
| The QuickPick IMAC kits provide a fast and simple means for recombinant histidine-tagged (His-tag) protein purification based on metal affinity using Ni2+. Immobilized metal ion affinity chromatography (IMAC) was originally introduced as a method for group separation, but today it represents one of the most important tools for the single-step purification of proteins based on polyhistidine tags. The principle of the mechanism is based on the interaction between a metal ion coordinated to a covalently bound chelating ligand and a His-tag protein.
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QuickPick™ CM
62201 • weak cation exchange kit for proteins, 8 preps 62211 • weak cation exchange kit for proteins, 48 preps
QuickPick™ DEAE
62101 • weak anion exchange kit for proteins, 8 preps 62111 • weak anion exchange kit for proteins, 48 preps
| The QuickPick™ CM and DEAE kits are based on magnetic cellulose particles coated with carboxymethyl (CM) groups or diethylaminoethyl (DEAE) groups. Different types of proteins will bind to the coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups. Typically a mixture of numerous proteins, for example cell lysate, is used as a sample with ion exchangers. After unbound molecules are washed away, the composition of the buffer is changed by altering the pH and salt concentration to release the molecule(s) of interest from the particles.
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Most of the QuickPick™ kits are scalable ("SML" = Small-Medium-Large) meaning that the amounts of reagents used are directly proportional to the sample size. The number of preparations for a kit is calculated for a "standard" sample size and may this vary depending on the actual sample sizes used.
62301 • metal affinity kit for proteins, 8 preps
62311 • metal affinity kit for proteins, 48 preps
62201 • weak cation exchange kit for proteins, 8 preps
62211 • weak cation exchange kit for proteins, 48 preps
QuickPick™ DEAE
62101 • weak anion exchange kit for proteins, 8 preps
62111 • weak anion exchange kit for proteins, 48 preps