Bioauxilium THUNDER™ TR-FRET Cell Signaling Assay Kits
Bioauxilium THUNDER™ TR-FRET Cell Signaling Assay Kits

Thunder TR-FRET signaling assays : now you have an alternative : BioAuxilium

제품에 대한 문의|주문은 biotools@icloud.com으로

Why choose TR-FRET assays from BioAuxilium?

Better components

       Carefully valdiated antibodies

        Optimal donor/acceptor fluorophores
        Optimized assay components (e.g. buffers)

Rigorous validation

        All kits are validated on lysates from activated cells
        Technical datasheets contain validation data

Lot-to-lot consistency

        Kits are developed and validated in-house
        New lots are measured against existing lots to ensure lot-to-lot consistency

Affordability

        A superior, cost-effective alternative to ELISA kits
        30% cheaper than TR-FRET kits from big suppliers



The Thunder TR-FRET assay platform

The ThunderTM TR-FRET assay platform is based on the use of donor-acceptor fluorophores showing exceptional spectral compatibility and TR-FRET signal:

        A long-lifetime and highly stable Europium chelate (Eu) as the donor fluorophore
        A small, unique far-red fluorophore (FR) as the acceptor

In a sandwich immunoassay application, one antibody is labeled with the Eu chelate and the second antibody is labeled with the FR fluorophore. The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two fluorophores into close proximity. Excitation of the donor Eu triggers a FRET to the acceptor FR, which in turn emits a specific TR-FRET signal at 665 nm. Residual (non-transferred) energy from the Eu generates light at 615 nm. These long-lived fluorescent signals are read in a time-resolved manner to minimize assay interference, which increases assay performance and specificity.


Thunder TR-FRET assays