Units contain a lateral flow strip designed to detect a biotin and FITC (or FAM) labelled amplicon. Detection is performed in a sealed cartridge preventing cross-contamination from amplified products. U-star disposable units can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer. See U-Star guide (PDF 117 KB) for further information.
Product code: USTAR01
This product is intended for rapid detection of a specific nucleic acid or amplicon that derived from polymerase chain reaction (PCR), cross priming amplification(CPA), Loop mediated isothermal amplification (LAMP), or any other nucleic acid amplification technologies.
The Disposable Nucleic Acid Detection Device is packed with an immune-chromatographic detection strip. Detection is performed in a sealed cartridge preventing cross-contamination from amplified products. Preparation requires merely 1-2 min and results appear in 10-15 min. In contrast to traditional electrophoresis, it offers a rapid, cross-contamination proof, non-toxic and instrument-free method for the visualization of nucleic acid amplification products.
If the Disposable Nucleic Acid Detection Device is not purchased along with an Ustar amplification kit, or if using amplification methods other than those provided by Ustar, primers and probes must be appropriately labeled to ensure compatibility with the detection strip. Generally, one primer/probe must be labeled with 5-Carboxyfluorescein (5-FAM) or Fluorescein isothiocyanate(FITC), and one primer/probe must be labeled with Biotin. To ensure capture of the target nucleic acid on the T-line, FITC and Biotin must both be incorporated, via hybridization or primer extension, into the target amplicon or nucleic acid. The control line appears irrespective of amplification and indicates that the strip is functioning properly.
1. Minimized the Risk of Nosocomial Infections
2. Easy Operation, Volume Control
3. Improved User Experience
4. Instrument-free and Cost-Effectiveness
5. Honored with iF product design award 2014