With this protocol, you can perform high-quality nucleic acid purifications using magnetic beads and the Opentrons Magnetic Module. This protocol contains flexible parameters that you can customize for many different magnetic bead and nucleic acid types. Use this setup to rapidly iterate and optimize your magbead-based workflows! You can use any magnetic beads you prefer with this protocol, but we have included some reagent recommendations in the Materials Needed section below to help you get started. For more detailed information on how to use this protocol, please see our Technical Note. -- Opentrons OT-2 -- Opentrons Magnetic Module -- Opentrons OT-2 Run App (Version 3.1.2 or later) -- 200uL or 300 uL Tiprack (Opentrons tips suggested) -- 12-row automation-friendly trough -- BioRad HardShell 96-Well PCR Plate -- Magnetic Beads (Looking for a kit? We recommend trying Omega Bio-tek Mag-Bind® TotalPure NGS) -- Ethanol -- Elution Buffer (Typically 10 mM Tris pH 8.0, TE Buffer, or nuclease-free water) Using the customization fields below, set up your protocol as follows: Pipette: Specify your pipette. We recommend using a p50 or p300 multi- or single-channel. Pipette Mount: Specify which mount (left or right) your pipette is on. Sample number: Customize the number of samples to run per protocol. A multiple of 8 is recommended when you are using a multichannel pipette. Sample volume: Specify the starting volume (in uL) of the input sample. Bead Ratio: Customize the ratio of beads for left or right side size-selection of fragments. The default bead ratio is 1.8x the input sample volume. Elution Volume: Specify the final volume (in uL) to elute the purified nucleic acid. The Opentrons MagDeck supports elution volumes above 10 µL. Incubation Time: Specify the amount of time (in minutes) that the bead solution and input sample interact. Settling Time: Specify the amount of time (in minutes) needed to pellet the beads. Higher volumes may require a longer settling time. Drying Time: Specify the drying time (in minutes) needed after wash steps.
NGS Cleanup and Size Selection with Omega Bio-tek Mag-Bind® TotalPure NGS
With this protocol, you can perform high-quality nucleic acid purifications using Omega Bio-tek Mag-Bind® TotalPure NGS magnetic beads and the Opentrons Magnetic Module. This setup yields high quality PCR product and other nucleic acids without the use of centrifugation or vacuum separation. This kit is widely used in NGS cleanup for its affordability and simplicity. It is also well-adapted for nucleic acid size selection by varying bead ratios for the isolation of a wide array of fragment sizes. For more detailed information on how to use this protocol, please see our Application Note. To purchase tips, reagents, or our Magnetic Module, please visit our online store or contact our Sales team at info@opentrons.com. Omega Bio-tek Mag-Bind® TotalPure NGS Kit Opentrons OT-2 Opentrons Magnetic Module Opentrons OT-2 Run App (Version 3.1.2 or later) 200uL or 300 uL Tiprack (Opentrons tips suggested) 12-row automation-friendly trough BioRad HardShell 96-Well PCR Plates Ethanol Elution Buffer (Typically 10 mM Tris pH 8.0, TE Buffer, or nuclease-free water) Using the customization fields below, set up your protocol as follows: Pipette: Specify your pipette. We recommend using a p50 or p300 multi- or single-channel. Pipette Mount: Specify which mount (left or right) your pipette is on. Sample number: Customize the number of samples to run per protocol. A multiple of 8 is recommended when you are using a multichannel pipette. Sample volume: Specify the starting volume (in uL) of the input sample. Bead Ratio: Customize the ratio of beads for left or right side size-selection of fragments. The default bead ratio is 1.8x the input sample volume. Elution Volume: Specify the final volume (in uL) to elute the purified nucleic acid. The Opentrons MagDeck supports elution volumes above 10 µL. Make sure to add reagents to your labware before placing it on the deck! You can see where to place your reagents below.
The Arcis sample preparation reagents will be available
Daresbury, UK, 09 July 2019: Arcis Biotechnology (“Arcis”), the nucleic acid sample preparation solution provider, today announced it has signed a commercial distribution agreement with Opentrons, the developer of affordable pipetting robots. The agreement allows Opentrons to directly distribute Arcis’ sample preparation kits to laboratories globally, providing scientists with a complete, accessible and automated solution for high throughput RNA/DNA preservation and preparation.
Arcis’ two-step, rapid nucleic acid extraction and preservation technology enables researchers to work with a wide range of samples to prepare RNA and DNA for downstream analysis in under three minutes, without the use of beads, spin columns, harsh solvents or heat-to-prepare samples. Under the terms of the agreement, Arcis’ novel product portfolio will be made available on the Opentrons OT-2 platform, an affordable lab robot with integrated electronic pipettes, designed to make automating lab protocols accessible to more laboratories. The collaboration provides researchers with an automated and high-throughput solution for sample preparation workflows.
Peter Whitehurst, Chief Executive Officer, Arcis Biotechnology, said: “After receiving feedback from scientists wanting to use Arcis reagents to process even more samples, we are proud to demonstrate automated and affordable solutions for RNA and DNA sample preparation for a breadth of applications.” He added: “We look forward to working with Opentrons, a partner with both a powerful engineering team and a customer-centric commercial approach.”
Will Canine, Co-Founder and Chief Product Officer, Opentrons, commented: “Arcis’ reagent-only solution is a compelling offering for biologists, but when you automate that protocol, it offers a high-throughput sample preparation workflow for every molecular biology laboratory. This collaboration with Arcis demonstrates our commitment to support scientists’ day-to-day workflow and to ultimately speed up scientific discovery.”
Nucleic Acid Purification with Magnetic Beads
With this protocol, you can perform high-quality nucleic acid purifications using magnetic beads and the Opentrons Magnetic Module. This protocol contains flexible parameters that you can customize for many different magnetic bead and nucleic acid types. Use this setup to rapidly iterate and optimize your magbead-based workflows! You can use any magnetic beads you prefer with this protocol, but we have included some reagent recommendations in the Materials Needed section below to help you get started. For more detailed information on how to use this protocol, please see our Technical Note. -- Opentrons OT-2 -- Opentrons Magnetic Module -- Opentrons OT-2 Run App (Version 3.1.2 or later) -- 200uL or 300 uL Tiprack (Opentrons tips suggested) -- 12-row automation-friendly trough -- BioRad HardShell 96-Well PCR Plate -- Magnetic Beads (Looking for a kit? We recommend trying Omega Bio-tek Mag-Bind® TotalPure NGS) -- Ethanol -- Elution Buffer (Typically 10 mM Tris pH 8.0, TE Buffer, or nuclease-free water) Using the customization fields below, set up your protocol as follows: Pipette: Specify your pipette. We recommend using a p50 or p300 multi- or single-channel. Pipette Mount: Specify which mount (left or right) your pipette is on. Sample number: Customize the number of samples to run per protocol. A multiple of 8 is recommended when you are using a multichannel pipette. Sample volume: Specify the starting volume (in uL) of the input sample. Bead Ratio: Customize the ratio of beads for left or right side size-selection of fragments. The default bead ratio is 1.8x the input sample volume. Elution Volume: Specify the final volume (in uL) to elute the purified nucleic acid. The Opentrons MagDeck supports elution volumes above 10 µL. Incubation Time: Specify the amount of time (in minutes) that the bead solution and input sample interact. Settling Time: Specify the amount of time (in minutes) needed to pellet the beads. Higher volumes may require a longer settling time. Drying Time: Specify the drying time (in minutes) needed after wash steps.
NGS Cleanup and Size Selection with Omega Bio-tek Mag-Bind® TotalPure NGS
With this protocol, you can perform high-quality nucleic acid purifications using Omega Bio-tek Mag-Bind® TotalPure NGS magnetic beads and the Opentrons Magnetic Module. This setup yields high quality PCR product and other nucleic acids without the use of centrifugation or vacuum separation. This kit is widely used in NGS cleanup for its affordability and simplicity. It is also well-adapted for nucleic acid size selection by varying bead ratios for the isolation of a wide array of fragment sizes. For more detailed information on how to use this protocol, please see our Application Note. To purchase tips, reagents, or our Magnetic Module, please visit our online store or contact our Sales team at info@opentrons.com. Omega Bio-tek Mag-Bind® TotalPure NGS Kit Opentrons OT-2 Opentrons Magnetic Module Opentrons OT-2 Run App (Version 3.1.2 or later) 200uL or 300 uL Tiprack (Opentrons tips suggested) 12-row automation-friendly trough BioRad HardShell 96-Well PCR Plates Ethanol Elution Buffer (Typically 10 mM Tris pH 8.0, TE Buffer, or nuclease-free water) Using the customization fields below, set up your protocol as follows: Pipette: Specify your pipette. We recommend using a p50 or p300 multi- or single-channel. Pipette Mount: Specify which mount (left or right) your pipette is on. Sample number: Customize the number of samples to run per protocol. A multiple of 8 is recommended when you are using a multichannel pipette. Sample volume: Specify the starting volume (in uL) of the input sample. Bead Ratio: Customize the ratio of beads for left or right side size-selection of fragments. The default bead ratio is 1.8x the input sample volume. Elution Volume: Specify the final volume (in uL) to elute the purified nucleic acid. The Opentrons MagDeck supports elution volumes above 10 µL. Make sure to add reagents to your labware before placing it on the deck! You can see where to place your reagents below.
The Arcis sample preparation reagents will be available
Daresbury, UK, 09 July 2019: Arcis Biotechnology (“Arcis”), the nucleic acid sample preparation solution provider, today announced it has signed a commercial distribution agreement with Opentrons, the developer of affordable pipetting robots. The agreement allows Opentrons to directly distribute Arcis’ sample preparation kits to laboratories globally, providing scientists with a complete, accessible and automated solution for high throughput RNA/DNA preservation and preparation.
Arcis’ two-step, rapid nucleic acid extraction and preservation technology enables researchers to work with a wide range of samples to prepare RNA and DNA for downstream analysis in under three minutes, without the use of beads, spin columns, harsh solvents or heat-to-prepare samples. Under the terms of the agreement, Arcis’ novel product portfolio will be made available on the Opentrons OT-2 platform, an affordable lab robot with integrated electronic pipettes, designed to make automating lab protocols accessible to more laboratories. The collaboration provides researchers with an automated and high-throughput solution for sample preparation workflows.
Peter Whitehurst, Chief Executive Officer, Arcis Biotechnology, said: “After receiving feedback from scientists wanting to use Arcis reagents to process even more samples, we are proud to demonstrate automated and affordable solutions for RNA and DNA sample preparation for a breadth of applications.” He added: “We look forward to working with Opentrons, a partner with both a powerful engineering team and a customer-centric commercial approach.”
Will Canine, Co-Founder and Chief Product Officer, Opentrons, commented: “Arcis’ reagent-only solution is a compelling offering for biologists, but when you automate that protocol, it offers a high-throughput sample preparation workflow for every molecular biology laboratory. This collaboration with Arcis demonstrates our commitment to support scientists’ day-to-day workflow and to ultimately speed up scientific discovery.”